Enhanced protein identification using graphite-modified MALDI plates for offline LC-MALDI-MS/MS bottom-up proteomics
نویسندگان
چکیده
منابع مشابه
The Power of LC MALDI: Identification of Proteins by LC MALDI
The Applied Biosystems 4700 Proteomics Analyzer with GPS ExplorerTM Software enables a fully automated and advanced LC MALDI MS/MS workflow for protein identification from complex mixtures. Novel MS peak detection identifies the presence of all peptides including those of closely related composition or isobaric peptides. Additionally, MS/MS spectra are collected at peak maxima increasing assay ...
متن کاملThe reproducibility of phospholipid analyses by MALDI-MSMS.
The identification of phosphocholine and phosphoethanolamine lipids by MALDI TOF/TOF, including characterisation of the headgroup and delineation of the acyl chain at each position of the glycerol backbone, has been explored using lipids representative of each type. The relative intensities of fragments involving the neutral loss of one or other of the acyl chains from ion adducts of 1-palmitoy...
متن کاملAn Automated LC–MALDI FT-ICR MS Platform for High-Throughput Proteomics
2 Introduction Post-genomic biology is marked by the desire to study cells, cellular processes and disease at the protein level. However, such studies are considerably more difficult to perform than their genomic counterparts because RNA processing, proteolytic activation and many post-translational modifications (PTMs) can result in the production of numerous proteins from a single gene. Addit...
متن کاملMALDI Imaging Mass Spectrometry (MALDI-IMS)—Application of Spatial Proteomics for Ovarian Cancer Classification and Diagnosis
MALDI imaging mass spectrometry (MALDI-IMS) allows acquisition of mass data for metabolites, lipids, peptides and proteins directly from tissue sections. IMS is typically performed either as a multiple spot profiling experiment to generate tissue specific mass profiles, or a high resolution imaging experiment where relative spatial abundance for potentially hundreds of analytes across virtually...
متن کاملIdentification of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry.
Direct mass spectrometric analysis of complex biological samples is becoming an increasingly useful technique in the field of proteomics. Matrix-assisted laser desorption/ionization mass spectroscopy (MALDI-MS) is a rapid and sensitive analytical tool well suited for obtaining molecular weights of peptides and proteins from complex samples. Here, a fast and simple approach to cellular protein p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2018
ISSN: 0003-2697
DOI: 10.1016/j.ab.2018.01.002